Hygeia journal for drugs and medicines


Simultaneous Multicomponent Analysis of Ampicillin and Probenecid in Pharmaceutical formulation by Reverse Phase High Performance Liquid Chromatography

ThomasKurian*, Jose Kurien, Helen William and Bijumol C.

College of Pharmaceutical Sciences,Govt. Medical College, Kottayam, Kerala-686008.

Article history: Received: 10 June2012,   revised: 14July, 2012, accepted:5August 2012, Available online:10 October 2012


Plan:ReversePhase High Performance Liquid Chromatographic method for Ampicillin andProbenecid described

Prologue: Ampicillin trihydrate is anantibiotic active against mainly gram positive bacteria and some gram negativebacteria. Probenecid is a uricosuric agent used in gout therapy. Thepresent study aims at the development of Reverse phase High Performance LiquidChromatography method for simultaneous estimation of Ampicillin and Probenecidin combined dosage formulations.

Methodology: The HPLC system used consists ofWaters 501 pump and a Waters 486 tunable wave length UV-visible detector. Thedata station was computer controlled using base line 810 software whichincludes an integrator and a recorder. The mobile phase used was sodium acetatesolution (50mm): acetonitrile (75:25). The UV response was measured at 235nm.The column used was Reverse phase(5 micron) 25cm x4.6mm.

Outcome: The method provided adequate accuracy and precision  .The developed RP-HPLC method can be used forthe routine analysis of Ampicillin and Probenecid in combination.

 Keywords: Ampicillin,Probenecid, Reverse phase, High performance liquid chromatography

For Correspondence:

 E-mail: thoku_ply@yahoo.com.


Hygeia.J.D.Med. Vol.4 (2),Oct. 2012

© 2012, Hygeia journal for drugs and medicines, allrights reserved.  22293590, 0975 6221 


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