Call for papers for the forthcoming issue of Hygeia.J.D.Med.10 (1) July 2018.

Abstract

Simultaneous Multicomponent Analysis of Ampicillin and Probenecid in Pharmaceutical formulation by Reverse Phase High Performance Liquid Chromatography

ThomasKurian*, Jose Kurien, Helen William and Bijumol C.

College of Pharmaceutical Sciences,Govt. Medical College, Kottayam, Kerala-686008.

Article history: Received: 10 June2012,   revised: 14July, 2012, accepted:5August 2012, Available online:10 October 2012

Abstract:

Plan:ReversePhase High Performance Liquid Chromatographic method for Ampicillin andProbenecid described

Prologue: Ampicillin trihydrate is anantibiotic active against mainly gram positive bacteria and some gram negativebacteria. Probenecid is a uricosuric agent used in gout therapy. Thepresent study aims at the development of Reverse phase High Performance LiquidChromatography method for simultaneous estimation of Ampicillin and Probenecidin combined dosage formulations.

Methodology: The HPLC system used consists ofWaters 501 pump and a Waters 486 tunable wave length UV-visible detector. Thedata station was computer controlled using base line 810 software whichincludes an integrator and a recorder. The mobile phase used was sodium acetatesolution (50mm): acetonitrile (75:25). The UV response was measured at 235nm.The column used was Reverse phase(5 micron) 25cm x4.6mm.

Outcome: The method provided adequate accuracy and precision  .The developed RP-HPLC method can be used forthe routine analysis of Ampicillin and Probenecid in combination.

 Keywords: Ampicillin,Probenecid, Reverse phase, High performance liquid chromatography

For Correspondence:

 E-mail: thoku_ply@yahoo.com.

Rid.G-9465-2012

Hygeia.J.D.Med. Vol.4 (2),Oct. 2012

© 2012, Hygeia journal for drugs and medicines, allrights reserved.  22293590, 0975 6221 

Hygeia J. D. Med.





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