Method Development and validation of Levosalbutamol sulphate by Derivative Spectroscopy
Hygeia.J.D.Med. 2013; 5(1):84-89
Nyola Narendra*1, 2, GovindaSamy Jeyabalan1
1 Department of Pharmaceutical Analysis,Alwar Pharmacy College, Alwar, India.
2. Department of Pharmaceutical Sciences,Shridhar University, Pilani, India
Article history: Received: 2 January 2013, revised: 29 January 2013, accepted: 23 February 2013, Available online:3 April 2013
Plan: An analytical method for estimation of levosalbutamol in bulk and pharmaceutical formulation.
Preface: The developed method is simple, fast and accurate and can be used for routine analysis of Market formulations.
Methodology: In this study zero, first and second order derivative spectrophotometric method were developed for the estimation of Levosalbutamol sulphate. In zero order spectrophotometery, absorbance value was measured at 277 nm. In first derivative spectrophotometry amplitudes were measured at 233 nm. In second derivative spectrophotometry amplitudes were measured at 237 nm. Calibration curves were linear between the concentration range of 20-60µg/ml, 5-30µg/ml and 35-60µg/ml respectively. The % RSD value is less than 2% and the recovery were near 100%for all methods.
Outcome: All the developed methods were applied on pharmaceutical formulations.
Key words: Spectrophotometric method, Levosalbutamol sulphate, Derivative spectroscopy.
Nyola Narendra, Govinda Samy Jeyabalan. Method Development and validation of Levosalbutamol sulphate byDerivative Spectroscopy.Hygeia.J.D.Med. 2013; 5(1):84-89.Available at http://www.hygeiajournal.com / Article ID- Hygeia.J.D.Med/92/13
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Hygeia.J.D.Med. Vol.5 (1), April 2013
© 2013, Hygeia journal for drugs and medicines,
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Researcher ID: C-2496-2013