16S rDNA Phylogenetic Analysis of Actinomycetes Isolated from Marine Environment Associated with Antimicrobial Activities
Hygeia.J.D.Med.5 (2) 43-50
S.Deepa*, K.Kanimozhi and A.Panneerselvam
PG and Research Dept. of Botany and Microbiology, A.V.V.M Sri Pushpam College, (Autonomous), Poondi- 613503, Thanjavur, Tamilnadu, India.
Article history: Received: 2 July 2013, revised: 20 July 2013, accepted: 11 August 2013, Available online: 10 October 2013
Article ID- Hygeia.J.D.Med/108/13.
Plan: Isolate and characterize the antimicrobial actinomycetes from sediments of salt pan region of Vedharanyam, located in the Nagapattinam District, Tamil Nadu, India.
Methodology: The salt pan soil samples were collected The Physico - chemical parameters of soil sample were analyzed. Totally 16 actinomycete strains were isolated. The isolated strains were identified based on the morphological, biochemical, and physiological characteristics. All the 16 actinomycetes were selected for antimicrobial activity. Totally 16 actinomycetes strains were isolated. Antifungal activity was determined against two fungal pathogenic such as Aspergillus niger, Fusarium moniliforme. Streptomyces griseoflavus SDAP101 showed maximum level of inhibition against Aspergillus niger followed by Fusarium moniliforme. Molecular characterization of Streptomyces griseoflavus SDAP101were evaluated by PCR amplification of 16SrRNA gene. The genomic DNA and amplified products were separated in agarose gel and the16SrDNA gene of Streptomyces griseoflavusSDAP101species isolated from soil was partially sequenced using specific 16SrDNA sequence primer. The phylogenetic analysis revealed that its closest neighbor was EU827474.1. The16SrDNA secondary structure and the restriction sites of SDAP101 were predicted using Genebee online software respectively.
Outcome: The isolation, characterization of the rare actinomycetes from the saltpan region will be useful for the discovery of the novel bioactive metabolites that are effective against wide range of pathogens
Key words: Streptomyces sp. SDAP101, antibacterial activity, molecular taxonomy, phylogenetic analysis.
S.Deepa*, K.Kanimozhi and A.Panneerselvam. 16S rDNA Phylogenetic Analysis of Actinomycetes Isolated from Marine Environment Associated with Antimicrobial Activities. Hygeia.J.D.Med. 2013; 5(2): page.43.-50. Available from http://www.hygeiajournal.com
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