Stability Indicating RP-HPLC method for the Ceftaroline Fosamil acetate
Hygeia.J.D.Med.5 (2) 97-103
Lalit Kumar Sharma 1*,Narendra Nyola 1, Shashi Kant2, and G.Jeyabalan1
1. Departmentof Pharmaceutical Chemistry, Alwar Pharmacy College, Alwar
2. Department of Analytical R&D,Jubilant Chemsys Ltd., Noida
Article history: Received: 27 July 2013, revised: 4 September 2013, accepted: 9 September 2013, Available online: 3 October 2013
Plan: Stability indicating RP-HPLCmethod for the ceftaroline fosamil acetate.
Preface: The developed method is simple, fast and accurate and can be used for routine analysis of Market formulations.
Methodology: Stability indicating reverse phase high performance liquid chromatography method was developed and validated for the analysis of ceftaroline fosamil in bulk form. Chromatographic separation was achieved on aX- Bridge shield RP (4.6×100mm, 5µ) column, maintained at 300c with a mobile phase consisting of acetonitrile and ammonium dihydrogen orthophosphate buffer in the ratio of 10: 90. The mobile phase pumped at a rate of 1ml/ min. and the detection was carried out at 242.6nm by PDA detector. The retention time was obtained 4.312.The peak area plot was linear over theconcentration range of 50µg/ml to 250µg/ml. The different experimental parameters affecting the stability were optimized. The method was validated for accuracy, precision, specificity, robustness, LOD and LOQ in accordance with International Conference on Harmonization (ICH) guidelines.
Outcome: The proposed method was successfully applied for the analysis of ceftaroline fosamil in bulk form.
Keywords: Ceftaroline Fosamil, RP-HPLCmethod, Validation.
Lalit Kumar Sharma, Narendra Nyola, Shashi Kant,G.Jeyabalan. Stability Indicating RP-HPLCmethod for the Ceftaroline Fosamil acetate Hygeia.J.D.Med 2013; 5(2):97-103,.Available from http://www.hygeiajournal.com / Article ID- Hygeia.J.D.Med/114/13.
Chromatogram of CeftarolineFosamil
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