Preliminary study on the altered expression of 3β HSD gene in rat testis after Amlodipine and its modification by Astercantha longifolia seed extract
Hygeia.J.D.Med.6 (1) 2014; 6-11.
1. College of Pharmaceutical Sciences, Medical College, Kozhikode, Kerala, India 673008.
2. College of Pharmaceutical Sciences, Medical College, Thiruvananthapuram, Kerala, India 695011.
Shini Dominic 1* and V. Padmaja 2
Article history: Received: 11 November 2013, revised: 10 January 2014, accepted: 17 February 2014, Available online: 3 April 2014
Plan: To verify the effect of Amlodipine on 3 β HSD gene expression in rat testis and its modification upon stopping the drug / co administration of Astercantha longifolia seed extract.
Preface: Though a few data are available on the potential of Amlodipine to adversely affect and that of Astercantha longifolia seeds to potentiate the male reproductive parameters, no studies are available on the effect of these on gene expression.
Methodology: Different groups of rat were treated orally with Amlodipine and Amlodipine plus Astercantha longifolia seed extract. After treatment the Amlodipine treated group was kept untreated for recovery. Finally total RNA from all groups were extracted and PCR for 3 β HSD gene was performed and products were analyzed by gel electrophoresis.
Outcome: Amlodipine treated groups showed down regulation of 3 β HSD expression but the same could be reversed after the recovery period of about 67 days. In groups treated with Amlodipine and Astercantha longifolia seed extract, 3 β HSD expression was up regulated in a dose dependent manner. It may be concluded that as 3 β HSD is involved in the conversion of cholesterol to testosterone, the decrease / increase in testosterone level may be due to the down / up regulation of 3 β HSD expression .
Key words: Amlodipine, Astercantha longifolia, 3 β HSD gene
For Correspondence: firstname.lastname@example.org
Researcher ID: C-3510-2013
Citation: Shini Dominic* and V. Padmaja. Preliminary study on the altered expression of 3β HSD gene in rat testis after Amlodipine and its modification by Astercantha longifolia seed extract. Hygeia.J.D.Med 2014; 6(1):6-11. Available from http://www.hygeiajournal.com / Article ID- Hygeia.J.D.Med/116/14. DOI: 10.15254/H.J.D.Med.6.2014.116
Expression analysis of β HSD in treated groups
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