Hygeia.J.D.Med.8(2) November 2016 - May 2017

Abstract

Preliminary study on the altered expression of 3β HSD gene in rat testis after Amlodipine and its modification by Astercantha longifolia seed extract

Hygeia.J.D.Med.6 (1) 2014; 6-11.

Shini Dominic 
1* and V. Padmaja 2


1. College of Pharmaceutical Sciences, Medical College, Kozhikode, Kerala, India 673008.
2. College of Pharmaceutical Sciences, Medical College, Thiruvananthapuram, Kerala, India 695011.

 

Article history: Received: 11 November 2013,   revised: 10 January 2014, accepted: 17 February 2014, Available online: 3 April 2014

 

Plan:  To verify the effect of Amlodipine on 3 β HSD gene expression in rat testis and its modification upon stopping the drug / co administration of Astercantha longifolia seed extract.

PrefaceThough a few data are available on the potential of Amlodipine to adversely affect and that of Astercantha longifolia seeds to potentiate the male reproductive parameters, no studies are available on the effect of these on gene expression.

Methodology: Different groups of rat were treated orally with Amlodipine and Amlodipine plus Astercantha longifolia seed extract. After treatment the Amlodipine treated group was kept untreated for recovery. Finally total RNA from all groups   were extracted and PCR for 3 β HSD gene was performed and products were analyzed by gel electrophoresis.

Outcome: Amlodipine treated groups showed down regulation of 3 β HSD expression but the same could be reversed after the recovery period of about 67 days. In groups treated with Amlodipine and Astercantha longifolia seed extract, 3 β HSD expression was up regulated in a dose dependent manner. It may be concluded that as 3 β HSD is involved in the conversion of cholesterol to testosterone, the decrease / increase in testosterone level may be due to the down / up regulation of  3 β HSD expression .

Key words: Amlodipine, Astercantha longifolia, 3 β HSD gene



For Correspondence:
 shinidm@yahoo.com    
Researcher ID: C-3510-2013    

Citation: Shini Dominic* and V. Padmaja. Preliminary study on the altered expression of 3β HSD gene in rat testis after Amlodipine and its modification by Astercantha longifolia seed extract. Hygeia.J.D.Med 2014; 6(1):6-11. Available from http://www.hygeiajournal.com / Article ID- Hygeia.J.D.Med/116/14.  DOI: 10.15254/H.J.D.Med.6.2014.116




                              Expression analysis of β HSD in treated groups

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Copyright@ 2014 Hygeia journal for drugs and medicines, 2229 3590, 0975 6221.
This is an open-access article distributed under the terms of the Creative Commons Attribution License.



Hygeia J. D. Med.





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