GC-MS Analysis of Bioactive components of Cordia retusa (Boraginaceae)
Hygeia.J.D.Med.6 (1) 2014; 12-19.
Murugesan Amudha*, Shanmugam Rani
Department of Pharmacy, FEAT, Annamalai University,Annamalai Nagar- 608 002, Chidambaram, Tamil Nadu, India.
Article History: Received: 5 December 2013, revised: 25 January 2014, accepted: 12 February 2014, Available online: 3 April 2014
Plan:The present study is structured to analyse the chemical constituents of the plant Cordia retusa (vahl.), belongs to the family Boraginaceae using GC-MS.
Methodology: The ethanolic crude extract of aerial part of plant C. retusa was analyzed. The GC Clarus 500 (Perkin Elmer) used in the investigation employed a column packed with Elite- 5MS (5%Diphenyl / 95% Dimethyl polysiloxane, 30mm x 0.25mm x0.25µmdf)and the components were separated using Helium (1 mL /min) as the carrier gas.The 2 µl sample extract injected into the instrument was detected by the Turbomass gold detector (Perkin Elmer) with the aid of the Turbo mass 5.2 software.
Outcome: Qualitative analysis of ethanolic crude extract of C.retusa by using GC MS showed that the presence of fourteen different phytochemical compounds. The components were recognized by comparing their retention time and fragmentation patterns with those data stored in the National Institute of Standards and Technology (NIST) library. The reported chief constituents are Alpha Amyrin, (1H) Naphthalenone,3,5,6,7,8,8a-hexahydro-4,8a-dimethyl-6-(1-methylethenyl)- and 9,19- Cycloergost -24(28)-en-3-ol, 4,14-dimethyl-,acetate, (3á,4à,5à).
Keywords: Cordia retusa (vahl.), GC-MS, NIST,Secondary metabolites
Funding: This study was supported by a research grant from the University Grant Commission, New Delhi by providing UGC-BSR fellowship to the author Ms. M.Amudha.
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Citation: Murugesan Amudha*, and Shanmugam Rani .GC-MS Analysis of Bioactive components of Cordia retusa (Boraginaceae). Hygeia.J.D.Med 2014; 6(1):12-19. Available from http://www.hygeiajournal.com /Article ID- Hygeia.J.D.Med/117/14.,DOI: 10.15254/H.J.D.Med.6.2014.117
The GC-MS chromatogram of ethanolic extract of C. retusa
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