Toxicokinetic and Tissue distribution studies of Mercury in an Ayurvedic preparation- Shila Sindur
Hygeia.J.D.Med.6 (1) 2014; 81-94.
Challa Srinivas Reddy1*,Umamahesh Balekari 2, Vemu Priyanka3, Jinugu Vidya Sagar4
1. Department of Pharmacognosy, Vaagdevi college of Pharmacy, Hanamkonda, India
2. Department of Pharmacognosy, University College of Pharmaceutical Sciences, Kakatiya University, Hanamkonda, Andhra Pradesh, India.
3. Department of Pharmacology, Vaagdevi College of Pharmacy, Hanamkonda, Andhra Pradesh, India.
4. Relisys Medical Devices Ltd, Hyderabad, Andhra Pradesh India.
Article history: Received: 5 December 2013, revised: 25 January 2014,accepted: 12 February 2014, Available online: 3 April 2014
Plan: Shila sindur, an ayurvedic preparation containing mercury as chief ingredient was investigated for toxicokinetics and tissue distribution studies in laboratory animals.
Methodology: Shila Sindur at three doses i.e., 50 mg/kg, 300 mg/kg and 1000 mg/kg was studied. Single and repeated dose administration was used for toxicokinetics and tissue distribution studies, respectively and evaluated by using toxicokinetic and tissue distribution parameters.
Outcome: Toxicokinetics studies revealed low plasma clearance with high half life, this correlates with higher affinity of mercury to plasma proteins. Higher doses showed wide distribution by increased area under curve and volume of distribution. A dose dependent elevation in mercury concentrations and organ damage was found in kidneys followed by liver, lungs,spleen and brain. At higher doses, shila sindur found toxic, at moderate doses stringent monitoring was recommended for use. Present study concludes that low dose Shila sindur was found safe in terms of toxicological and tissue distribution patterns.
Key words: Shila sindur, toxicokinetics,tissue distribution, mercury
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Citation: Challa Srinivas Reddy*,Umamahesh Balekari, Vemu Priyanka, Jinugu Vidya Sagar. Toxicokinetic and Tissue distribution studies of Mercury in an Ayurvedic preparation- Shila Sindur .Hygeia.J.D.Med 2014; 6(1):81-94.Available from http://www.hygeiajournal.com / Article ID- Hygeia.J.D.Med/125/14. D.O.I:10.15254/H.J.D.Med.6.2014.125.
Histopathology of liver
Control (A): Liver showing normal architecture
Low dose (B): Showing mild degenerative changes with few focal mono nuclear cell infiltration
Medium dose (C)showing mild-moderate degenerative changes with dilatation of hepatic venule with focal mono nuclear cell infiltration
High dose (D):showing dilated bile duct and portal tract with a proliferative changes and occasional necrotic changes
Histopathology of kidney
Control (A):Showing normal renal cortical parenchyma
Low dose (B):Showing normal renal cortical parenchyma with focal mild mono nuclearinfiltration in Bowman's capsule
Medium dose (C):Shows mild degenerative changes with mono nuclear infiltration in the renalparenchyma
High dose (D):Show diffuse infiltration of mono nuclear cells with focal necrotic debris
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