In Vitro Anticancer Activity of Papain Hydrolysates of Oyster Mushroom (Pleurotus ostreatus) Protein
Hygeia.J.D.Med.6(2) 2014; 1-4.
Sundaram Meignanalakshmi, Aarthi KS, Parthiban M and Palanisammi A
Departmentof Animal Biotechnology, Madras Veterinary College, Chennai-7
Plan: The present study has been undertaken to evaluate the in vitro anticancer activity of papain hydrolysates of Oyster mushroom (Pleurotus ostreatus) protein.
Preface: Oyster mushroom protein was isolated by phosphate buffer method and concentration of protein was found tobe 24mg/g of Oyster mushroom.
Methodology: The total protein was enzyme hydrolysed by papain (enzyme and substrate was added at a ratio of 1:5) In vitro anticancer activity was studied by Percentage cell inhibition in MCF-7 cellline using MTT assay. In vitro percentage cell viability was tested in vero cell line.
Outcome: Papain hydrolysates was found to be having highest percentage cell inhibition of 75.82% at 100 µg/ml, 89.83% at 500 µg/ml concentration respectively in MCF-7 cell line. Papain hydrolysates were found to behaving highest percentage cell viability of 97.23% at 100 µg/ml and 83.72% at 500 µg/ml concentration respectively.
Key words: Oyster mushroom, anticancer activity, Papain hydrolysates, MTT assay, Percentage cell inhibition, Percentage cell viability.
Received: 5 July 2014, Revised: 20 July 2014,Accepted: 30 July 2014, Available online: 10 October 2014
Department of AnimalBiotechnology, Madras Veterinary College, Chennai-7
Sundaram Meignanalakshmi, Aarthi KS,Parthiban M and Palanisammi A. In vitro anticancer activity of papainhydrolysates of Oyster mushroom (Pleurotusostreatus) protein. Hygeia.J.D.Med 2014;6(2):1-4. Available from http://www.hygeiajournal.com / Article ID-Hygeia.J.D.Med/130/14. doi:10.15254/H.J.D.Med.6.2014.130.
Cell inhibition % in MCF -7 Cell line by Total protein and Papain hydrolysates of Oyster mushroom protein
Cell viability % in Vero Cell line by Total protein and Papain hydrolysates of Oyster mushroom protein