Hygeia J. D.Med.9 (2) January 2018 - July 2018 ......... ......... Call for papers for the forthcoming issue of Hygeia.J.D.Med.10 (1) July 2018.

Abstract

In Vitro Anticancer Activity of Papain Hydrolysates of Oyster Mushroom (Pleurotus ostreatus) Protein

Hygeia.J.D.Med.6(2) 2014; 1-4.

Sundaram Meignanalakshmi, Aarthi KS, Parthiban M and Palanisammi A

Departmentof Animal Biotechnology, Madras Veterinary College, Chennai-7

Plan: The present study has been undertaken to evaluate the in vitro anticancer activity of papain hydrolysates of Oyster mushroom (Pleurotus ostreatus) protein.

Preface: Oyster mushroom protein was isolated by phosphate buffer method and concentration of protein was found tobe 24mg/g of Oyster mushroom.

Methodology: The total protein was enzyme hydrolysed by papain (enzyme and substrate was added at a ratio of 1:5) In vitro anticancer activity was studied by Percentage cell inhibition in MCF-7 cellline using MTT assay.  In vitro percentage cell viability was tested in vero cell line.

Outcome: Papain hydrolysates was found to be having highest  percentage cell inhibition of 75.82% at 100 µg/ml, 89.83% at 500 µg/ml  concentration respectively in MCF-7 cell line. Papain hydrolysates were found to behaving highest percentage cell viability of 97.23% at 100 µg/ml and 83.72% at 500 µg/ml concentration respectively.

Key wordsOyster mushroom, anticancer activity, Papain hydrolysates, MTT assay, Percentage cell inhibition, Percentage cell viability.

Received: 5 July 2014, Revised: 20 July 2014,Accepted: 30 July 2014, Available online: 10 October 2014

Correspondence:


Sundaram Meignanalakshmi

Department of AnimalBiotechnology, Madras Veterinary College, Chennai-7

Email: smeignanalakshmi@gmail.com


Sundaram Meignanalakshmi, Aarthi KS,Parthiban M and Palanisammi A. In vitro anticancer activity of papainhydrolysates of Oyster mushroom (Pleurotusostreatus) protein. Hygeia.J.D.Med 2014;6(2):1-4. Available from http://www.hygeiajournal.com / Article ID-Hygeia.J.D.Med/130/14. doi:10.15254/H.J.D.Med.6.2014.130.





Cell inhibition % in MCF -7 Cell line by Total protein and Papain hydrolysates of Oyster mushroom protein



Cell viability % in Vero Cell line by Total protein and Papain hydrolysates of Oyster mushroom protein




Hygeia J. D. Med.





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