Abstract
In vitro anti-inflammatory and cytotoxicity studies of two mangrove associated lichens, Dirinaria consimilis and Ramalina leiodea extracts
Hygeia.J.D.Med 2018; 10(1):16-26
DOI: 10.15254/H.J.D.Med.10.2018.174.
Vinay Bharadwaj Tatipamula* and Girija Sastry Vedula
Pharmaceutical Chemistry Department, University College of Pharmaceutical Sciences, Andhra University, Visakhapatnam – 530 003, Andhra Pradesh, India.
Plan: To evaluate the anti-inflammatory and cytotoxicity activity of different extracts from two different manglicolous lichens (Dirinaria consimilis and Ramalina leiodea).
Preface: Inflammation is the origin of several deadly diseases like cancer, atherosclerosis, Alzheimer’s and rheumatoid arthritis. From decades, lichen extracts and its metabolites are well known in treating inflammation and cancer.
Outcome: The outcome of protein denaturation method confirmed that the ethyl acetate and acetone extract of R. leiodea depicted better inhibitory profile against protein denaturation with IC50 values of 268 and 330 µg/mL Furthermore, the results of SRB assay showed that ethyl acetate and acetone extracts of both the lichens acts potently against MCF-7, DLD-1, HeLa and A549. Simultaneously, all the tested extracts depicted the low degree of specificity towards NHME, they are less toxic. Hence, further screening of these extracts may lead to the exploration of safe and potent anti-inflammatory and anticancer agents.
Keywords: Inflammation, cancer, protein denaturation method, SRB assay, cytotoxicity
Correspondence
Vinay Bharadwaj Tatipamula M.Pharm, Ph.D
Pharmaceutical Chemistry Department, University College of Pharmaceutical Sciences, Andhra University,
Visakhapatnam, Andhra Pradesh, India. – 530 003
vinaybharadwajt@gmail.com,
Mobile: +91-9640325675
ORCIDID: 0000-0002-9376-1911, Researcher id: L-8352-2018
Article History
Received: 15 April 2018,
Revised: 9 May 2018
Accepted: 28 May 2018,
Available online: 15 September 2018
Article citation
Vinay Bharadwaj Tatipamula and Girija Sastry Vedula. In vitro anti-inflammatory and cytotoxicity studies of two mangrove associated lichens, Dirinaria consimilis, and Ramalina leiodea extracts. Hygeia.J.D.Med 2018; 10(1):16-26.Available from http://www.hygeiajournal.com, DOI: 10.15254/H.J.D.Med.10.2018.174.