Toxicological Profiles of the Leaf Extracts of Wrightia arborea and Wrightia tinctoria
Hygeia J. D. Med. Vol.2 (1) 2010, 46-53
(ISSN 0975 6221)
Type of Article
Lakshmi devi .S1*, Madhu. C. Divakar2
1. Department of Pharmacognosy, Sri Ramakrishna College of Pharmacy,SRIPMS, Coimbatore,TamilNadu,India,641044
2.Crescent College of Pharmaceutical Sciences, Kannur, Kerala, India-670358
The research work was conducted with the leaf solvent extracts of Wrightia arborea and Wrightia tinctoria to make toxicological profiles by employing Brine Shrimp Assay method (BSA) (Artemia Salina LEACH).. The LC50 values were determined for both the plant solvent extracts respectively in mg/ml of active compounds and extracts. It was found that the leaf ethanolic and methanolic extracts were toxic for the Brine Shrimp Naupli. The results indicated that Wrightia tinctoria leaf ethanol (70%) extract and methanolic extract showed LC50 values of 471.604 and 517.038 mg/ml respectively .While the Wrightia arborea leaf ethanol (70%) extract and methanolic extracts showed LC50 values of 498.213 and 531.082 mg/ml respectively. The remaining solvent extracts showed no toxicity (as found more than 1000 mg/ml) in BSA method.
Key Words: Wrightia tinctoria, Wrightia arborea, Brine shrimp Assay (BSA), Toxicological profiles.
2. 2. Plant Material
2. 3. Preparation of Extract
2. 4. Preparation of Simulated Sea Water
2. 5 .Preparation of test sample
2. 6. Bioassay
3. Results and discussion
Determination of LC50 for 70% ethanolic extract of Wrightia tinctoriaand W.arborea
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