Phytochemical, HPTLC finger printing and antibacterial activity of Acacia nilotica (L.) Delile
Hygeia.J.D.Med.Vol.2 (2), 2010, 38-42.
Type of article: research article
Authors: R.Venkataswamy1, A.Doss2, H. Mohamed Mubarack2 , M.Sukumar1 .
1. Department of Pharmacognosy, Sri Ramakrishna College of Pharmacy , Coimbatore , Tamil Nadu , India .
2. Department of Microbiology, RVS College of Arts and Science, Sulur, Coimbatore, Tamil Nadu, India .
Received: 5 April 2010,
Revised: 16 May 2010,
Accepted: 22 June 2010,
Available online: 31 July 2010.
The leaves of Acacia nilotica (L.) Delile was extracted with methanol and aqueous medium and studied for in vitro antimicrobial property. The methanol extract was found to be most active against all the bacterial species tested except S.aureus. Active methanol extract was further studied for HPTLC fingerprint and by phytochemical analysis. HPTLC analysis confirmed segregation of four individual compounds with individual Rf values and peak area percentage. The results of phytochemical screening of extract revealed the presence of Tannins, Carbohydrates and Glycosides. This analysis revealed the high antibacterial activity in the methanol extract of Acacia nilotica (L.) Delile
Key words: Acacia nilotica (L.) Delile , HPTLC finger printing, methanol, MIC, microorganisms
2. Materials and methods
HPTLC finger printing.
Minimum Inhibitory Concentration
3. Results and discussion
HPTLC finger print of the methanolic extract of
Acacia nilotica (L.) Delile
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