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Evaluation of antibacterial activity of the methanol extract of the leaves of Adhatoda vasica on mastitis pathogens

Hygeia.J.D.Med. 2013;5(1):1-4.

Meignanalakshmi S, Vinoth Kumar S, Deepika J and Farida Begum I.

Department of Biotechnology, School of Bio engineering, SRM University, Kattankulathur-.India, 603 203

Article history: Received: 10 January 2013, revised: 4 February 2013, accepted: 15 February 2013, Available online: 3 April 2013

Abstract

Plan: The present study was undertaken to evaluate the antimicrobial activity of aqueous and methanol extracts of Adhatoda vasica against mastitis pathogens.

Methodology: Milk samples were collected from mastitis cows and five bacteria were isolated. Based on Grams staining and various biochemical tests, they were identified as Staphylococcus aureus, Streptococcus agalactiae, Klebsiella pneumoniae, Streptococcus dysgalactiae and Escherichia coli. Leaves of Adhatoda vasica was subjected to aqueous and methanol extraction. Antibacterial activity of the extracts was evaluated by disc diffusion method and zone of inhibition was measured.

Outcome: The methanol extract was found to be having significant antibacterial activity against Staphylococcus aureus, Streptococcus agalactiae, Klebsiella pneumonia,Streptococcus dysgalactiae and Escherichia coli with zone of inhibition 21.7±0.58 mm, 18.3±0.58 mm, 21.3±0.58, 18.3±0.58 and 28.3±0.58 mm respectively at 200 mg/ml concentration.

Keywords: Mastitis,Adhatoda vasica, antibacterial activity, methanol extract, disc diffusion

Meignanalakshmi S, Vinoth Kumar S, Deepika J and Farida Begum I. Hygeia.J.D.Med. 2013; 5(1):1-4.Available from http://www.hygeiajournal.com / Article ID- Hygeia.J.D.Med/81/13.