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Preliminary phytochemical analysis of methanolic extract of Tinospora cordifolia and its anti-bacterial action on E.coli cell division
Hygeia.J.D.Med. 2017; 9 (1):52-60
DOI:10.15254/H.J.D.Med.9.2017.164
Abhinay kumar BS1, Kumar, JR2, Murugesan Karthikeyan3, Ashok Gnanasekaran3, Akshay V4, Virkam Reddy5, Balasubramanian S6, Ravi raja S7 and Kanthesh M Basalingappa1*
1. Division of Molecular Biology, Department of Water and Health, Faculty of Life Sciences, Jagadguru Sri Shivarathreeswara (JSS) University, Mysore-570015,
2. Division of Biochemistry, Department of Water and Health, Faculty of Life Sciences, Jagadguru Sri Shivarathreeswara (JSS) University, Mysore-570015,
3. Senior Lecturer, Department of Microbiology, Faculty of Medicine, Quest International University Perak, Ipoh, Malaysia.
4. Department of Studies in Biochemistry, University of Mysore, Mysore 570006,
5. Professor, Department of Microbiology, Sri Lakshmi Narayana Institute of Medical Sciences, Puducherry-605502 and
6. Director of Research, Jagadguru Sri Shivarathreeswara (JSS) University, Mysore-570015.
7. Founder Chairman, Royal Research Foundation, Mysore-570028.
ABSTRACT
Plan: The study involves the antibacterial screening the methanolic extract of Tinospora cordifolia and its preliminary phytochemical analysis.
Preface: Bacterial infections have become increasingly difficult to treat because of multi-drug resistance. Although bacteria have over 200 specific protein targets for therapy, only three are most successfully exploited now. Bacterial cell division proteins are attractive targets for drug development. The "Filamenting temperature-sensitive mutant Z (FtsZ) protein is a major protein of the bacterial cell division machinery and is a potential target for therapy. Inhibition of FtsZ protein results in the inability of bacteria to divide and hence they die. Plant secondary metabolites have been shown to have antibacterial activities. However, their mechanism of action is not thoroughly investigated.
Methodology: In this study, we have used the methanolic extract of Tinospora cordifolia plant and tested its anti-microbial action by zone inhibition method.
Outcome: Although the T.cordifolia extract did not show high anti-bacterial activity by disc diffusion assay, the E.coli showed elongated structures in the presence of T.cordifolia extract suggesting that the extract is interfering with the bacterial cell division. HPLC analysis of the extract showed the presence of Berberin, which may be the active molecule in inhibiting FtsZ protein and subsequent cell division.
Key words: E.coli, Bacterial cell division, FtsZ, Berberine, Tinospora cordifolia.
Correspondence
Kanthesh M. Basalingappa MSc, PhD, Division of Molecular Biology
Faculty of Life SciencesJagadguru Sri Shivarathreeswara (JSS) University, SS Nagara, Mysuru-570015
kanthu4001@gmail.com
Article History: Received: 2 January 2017, Revised: 20 February 2017Accepted: 10 April 2017, Available online: 15 July 2017
Article citation
Abhinay Kumar BS, Kumar, JR, Murugesan Karthikeyan, Ashok Gnanasekaran, Akshay V, Vikram Reddy, Balasubramanian S, Ravi raja S and Kanthesh M Basalingappa* . Preliminary phytochemical analysis of methanolic extract of Tinospora cordifolia and its anti-bacterial action on E.colicell division. Hygeia.J.D.Med 2017; 9(1):52-60. Available from http://www.hygeiajournal.com , DOI: 10.15254/H.J.D.Med.9.2017.164
E.coli grown in the presence of 25 μg/ml T. cordifolia extract. The E.coli cells were elongated showing inhibition of cell division.